Description
Description: This assay employs a two-site sandwich ELISA to quantitate GJA5 in samples. An antibody specific for GJA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GJA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GJA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GJA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Gap junction alpha-5 protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described.The CX40 gene encodes a predicted 358-amino acid protein whose sequence is 82% identical to that of the rat and mouse CX40 protein. Northern blot analysis showed that CX40 mRNA is expressed as an approximately 3.3-kb transcript in ventricular myocardium. In immunofluorescence studies, CX40 localized to intercalated disc regions of the left ventricle, which join cardiac myocytes and contain gap junctions